Use of pour plate technique in tuberculocidal efficacy testing according to EN 14348 – a comparative study

Published:November 23, 2022DOI:


      To prevent spread of Mycobacterium tuberculosis (Mtb) effective disinfection strategies are essential. Tuberculocidal efficacy of disinfectants can be demonstrated by testing disinfectants in in-vitro tests, such as the well-established quantitative suspension test EN 14348 using M. terrae as a surrogate organism in European disinfectant testing.
      While in other European standard tests such as EN 13727 or EN 13624 use of pour plate technique is well established, in EN 14348 only spread plate technique is considered.
      In this study comparative experiments according to EN 14348 with M. terrae were conducted using a peracetic acid-based disinfectant. For cultivation either pour plate or spread plate technique was used.
      Differences in colony size and morphology were observed when comparing growth of M. terrae on pour versus spread plates. However, no significant differences in biocidal efficacy data were obtained when applying either spread plates or pour plates in the quantitative suspension test EN 14348 under both clean and dirty conditions.


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      1. Global tuberculosis report 2019; World Health Organisation, 2019, ISBN 978-92-4-156571-4

      2. Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of mycobactericidal activity of chemical disinfectants in the medical area including instrument disinfectants - Test methods and requirements (phase 2, step 1); German version EN 14348:2005

      3. European Committee for Standardization. European Standard EN 13727: Chemical disinfectants and antiseptics – Quantitative suspension test for the evaluation of bactericidal activity in the medical area – Test method and requirements (Phase 2/Step 1) EN 13727:2012+A2:2015, Brussels

      4. European Committee for Standardization. European Standard EN 13624: chemical disinfectants – quantitative suspension test for evaluation of fungicidal or yeasticidal activity in the medical area. Test method and requirements (phase 2, step 1). Brussels: ECS; 2013.

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