Manual for the detection of pathogen 2019-nCoV Ver.2.6.
|2× OneStep RT-PCR Buffer III|
|Primer (forward)||2.4 μM, 5′-AAATTTTGGGGACCAGGAAC-3′|
|Primer (reverse)||3.2 μM, 5′-TGGCAGCTGTGTAGGTCAAC-3′|
|Probe||0.4 μM, FAM-ATGTCGCGCATTGGCATGGA-TAMRA|
|10 x ROX||0.2×|
|PrimeScript RT enzyme Mix II|
|SpeedSTAR HS DNA Polymerase||0.25 U/μL|
Research use only 2019-novel coronavirus (2019-nCoV) real-time RT-PCR primer and probe information.
- Li Y.
- Xia L.
Conflict of interest statement
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- Research use only 2019-novel coronavirus (2019-nCoV) real-time RT-PCR primer and probe information.CDC, Atlanta, GA2020 (Available at:)[last accessed March 2020])
- Coronavirus disease 2019 (COVID-19): role of chest CT in diagnosis and management.Am J Roentgenol. 2020; https://doi.org/10.2214/AJR.20.22954
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