Efficacy of various disinfectants against SARS coronavirus

  • H.F. Rabenau
    Corresponding author. Tel.: +49 69 6301 5312; fax: +49 69 6301 83061.
    Institut für Medizinische Virologie, Klinikum der Johann Wolfgang Goethe-Universität Frankfurt, Paul-Ehrlich-Str. 40, 60596 Frankfurt, Germany
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  • G. Kampf
    Bode Chemie GmbH & Co., Scientific Affairs, Melanchthonstr. 27, 22525 Hamburg, Germany

    Institut für Hygiene und Umweltmedizin, Ernst-Moritz-Arndt Universität Greifswald, Walther-Rathenau-Str. 49a, 17489 Greifswald, Germany
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  • J. Cinatl
    Institut für Medizinische Virologie, Klinikum der Johann Wolfgang Goethe-Universität Frankfurt, Paul-Ehrlich-Str. 40, 60596 Frankfurt, Germany
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  • H.W. Doerr
    Institut für Medizinische Virologie, Klinikum der Johann Wolfgang Goethe-Universität Frankfurt, Paul-Ehrlich-Str. 40, 60596 Frankfurt, Germany
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      The recent severe acute respiratory syndrome (SARS) epidemic in Asia and Northern America led to broad use of various types of disinfectant in order to control the public spread of the highly contagious virus. However, only limited data were available to demonstrate their efficacy against SARS coronavirus (SARS-CoV). We therefore investigated eight disinfectants for their activity against SARS-CoV according to prEN 14476. Four hand rubs were tested at 30 s (Sterillium, based on 45% iso-propanol, 30% n-propanol and 0.2% mecetronium etilsulphate; Sterillium Rub, based on 80% ethanol; Sterillium Gel, based on 85% ethanol; Sterillium Virugard, based on 95% ethanol). Three surface disinfectants were investigated at 0.5% for 30 min and 60 min (Mikrobac forte, based on benzalkonium chloride and laurylamine; Kohrsolin FF, based on benzalkonium chloride, glutaraldehyde and didecyldimonium chloride; Dismozon pur, based on magnesium monoperphthalate), and one instrument disinfectant was investigated at 4% for 15 min, 3% for 30 min and 2% for 60 min [Korsolex basic, based on glutaraldehyde and (ethylenedioxy)dimethanol]. Three types of organic load were used: 0.3% albumin, 10% fetal calf serum, and 0.3% albumin with 0.3% sheep erythrocytes. Virus titres were determined by a quantitative test (endpoint titration) in 96-well microtitre plates. With all tested preparations, SARS-CoV was inactivated to below the limit of detection (reduction factor mostly ≥4), regardless of the type of organic load. In summary, SARS-CoV can be inactivated quite easily with many commonly used disinfectants.


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        • Guan Y.
        • Peiris J.S.
        • Zheng B.
        • et al.
        Molecular epidemiology of the novel coronavirus that causes severe acute respiratory syndrome.
        Lancet. 2004; 363: 99-104
        • Berger A.
        • Drosten C.
        • Doerr H.W.
        • Sturmer M.
        • Preiser W.
        Severe acute respiratory syndrome (SARS)—paradigm of an emerging viral infection.
        J Clin Virol. 2004; 29: 13-22
        • Fleck F.
        SARS virus returns to China as scientists race to find effective vaccine.
        Bull WHO. 2004; 82: 152-153
        • Anonymous
        Cluster of severe acute respiratory syndrome cases among protected healthcare workers—Toronto, Canada, April 2003.
        MMWR. 2003; 52: 433-436
        • Li H.L.
        • Feng X.R.
        • Dai L.
        • Yang Q.
        Epidemiologic investigation of nosocomial infection of SARS in medical staff of hospital.
        Di Yi Jun Yi Da Xue Xue Bao. 2004; 24: 355-358
        • Ho K.Y.
        • Singh K.S.
        • Habib A.G.
        • et al.
        Mild illness associated with severe acute respiratory syndrome coronavirus infections: lessons from a prospective seroepidemiologic study of healthcare workers in a teaching hospital in Singapore.
        J Infect Dis. 2004; 189: 642-647
        • Manocha S.
        • Walley K.R.
        • Russell J.A.
        Severe acute respiratory distress syndrome (SARS): a critical care perspective.
        Crit Care Med. 2003; 31: 2684-2692
        • Seto W.H.
        • Tsang D.
        • Yung R.W.H.
        • et al.
        Effectiveness of precautions against droplets and contact in prevention of nosocomial transmission of severe acute respiratory syndrome (SARS).
        Lancet. 2003; 361: 1519-1520
        • Kampf G.
        Mikrobielle Besiedlung der Hände und ihre epidemiologische Bedeutung.
        in: Kampf G. Händehygiene im Gesundheitswesen. Springer, Berlin2003: 29-64
        • Duan S.M.
        • Zhao X.S.
        • Wen R.F.
        • et al.
        Stability of SARS coronavirus in human specimens and environment and its sensitivity to heating and UV irradiation.
        Biomed Environ Sci. 2003; 16: 246-255
        • Rabenau H.F.
        • Cinatl J.
        • Morgenstern B.
        • Bauer G.
        • Preiser W.
        • Doerr H.W.
        Stability and inactivation of SARS coronavirus.
        Med Microbiol Immunol. 2005; 194: 1-6
        • Radun D.
        • Niedrig M.
        • Ammon A.
        • Stark K.
        SARS: retrospective cohort study among German guests of the hotel “M”, Hong Kong.
        Eurosurveillance. 2003; 8: 228-230
      1. Robert Koch-Institut. Empfehlung des Robert Koch-Institutes für die Hygienemaβnahmen und Infektionskontrolle bei Patienten mit Schwerem Akutem Respiratorischem Syndrom (SARS). Berlin: Robert Koch-Institut; 2003.

      2. WHO. First data on stability and resistance of SARS coronavirus compiled by members of WHO laboratory network. Geneva: WHO; 2003.

        • DGHM
        Liste der nach den “Richtlinien für die Prüfung chemischer Desinfektionsmittel” geprüften und von der Deutschen Gesellschaft für Hygiene und Mikrobiologie als wirksam befundenen Desinfektionsverfahren.
        mhp-Verlag, Wiesbaden2002
        • Drosten C.
        • Gunther S.
        • Preiser W.
        • et al.
        Identification of a novel coronavirus in patients with severe acute respiratory syndrome.
        N Engl J Med. 2003; 348: 1967-1976
        • Kärber G.
        Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche.
        Arch Exp Path Pharmak. 1931; 162: 480-487
        • Spearman C.
        The method of ‘right or wrong cases’ (constant stimuli) without Gauss's formulae.
        Br J Psychol. 1908; 2: 227-242
      3. prEN 14476. Chemical disinfectants and antiseptics. Virucidal quantitative suspension test for chemical disinfectants and antiseptics used in human medicine. Test method and requirements (phase 2, step 1). Brussels: Comité Européen de Normalisation; 2002.

        • Cinatl J.
        • Morgenstern B.
        • Bauer G.
        • Chandra P.
        • Rabenau H.
        • Doerr H.W.
        Glycyrrhizin, an active component of liquorice roots, and replication of SARS-associated coronavirus.
        Lancet. 2003; 361: 2045-2046
        • Cinatl J.
        • Morgenstern B.
        • Bauer G.
        • Chandra P.
        • Rabenau H.
        • Doerr H.W.
        Treatment of SARS with human interferons.
        Lancet. 2003; 362: 293-294
        • Kampf G.
        • Kramer A.
        Epidemiologic background of hand hygiene and evaluation of the most important agents for scrubs and rubs.
        Clin Microbiol Rev. 2004; 17: 863-893
        • Groupe V.
        • Engle C.C.
        • Gaffney P.E.
        Virucidal activity of representative antiinfective agents against influenza A and vaccinia virus.
        Appl Microbiol. 1955; 3: 333-336
        • Kampf G.
        • Rudolf M.
        • Labadie J-C.
        • Barrett S.P.
        Spectrum of antimicrobial activity and user acceptability of the hand disinfectant agent Sterillium Gel.
        J Hosp Infect. 2002; 52: 141-147
        • Cunningham C.H.
        The effect of certain chemical agents on the virus of Newcastle disease of chicken.
        Am J Vet Res. 1948; 9: 195-197
        • Bucca M.A.
        The effect of various chemical agents on eastern equine encephalomyelitis virus.
        J Bacteriol. 1956; 71: 491-492
        • Bond W.X.V.
        • Favero M.S.
        • Petersen N.J.
        Inactivation of hepatitis B virus by intermediate to high-level disinfectant chemicals.
        J Clin Microbiol. 1983; 18: 535-538
        • Kobayashi H.
        • Tsuzuki M.
        • Koshimizu K.
        Susceptibility of hepatitis B virus to disinfectants or heat.
        J Clin Microbiol. 1984; 20: 214-216
        • Payan C.
        • Pivert A.
        • Kampf G.
        • Ramon C.
        • Cottin J.
        • Lemarie C.
        Assessment of new chemical disinfectants for HBV virucidal activity in a cell culture model.
        J Hosp Infect. 2004; 56: S58-S63
        • Martin L.S.
        • Meoougal J.S.
        • Loskoski S.L.
        Disinfection and inactivation of the human T lymphotropic virus type III/lymphadenopathy associated virus.
        J Infect Dis. 1985; 152: 400-403
        • Spire B.
        • Barre-Sinoussi F.
        • Montagnier L.
        Inactivation of lymphadenopathy associated virus by chemical disinfectants.
        Lancet. 1984; 2: 899-901
        • Reiss I.
        • Borkhardt A.
        • Fussle R.
        • Sziegoleit A.
        • Gortner L.
        Disinfectant contaminated with Klebsiella oxytoca as a source of sepsis in babies.
        Lancet. 2000; 356: 310-311
        • Rutala W.A.
        • Weber D.J.
        Reprocessing endoscopes: United States perspective.
        J Hosp Infect. 2004; 56: S27-S39
        • Martiny H.
        • Floss H.
        • Zühlsdorf B.
        The importance of cleaning for the overall results of processing endoscopes.
        J Hosp Infect. 2004; 56: S16-S22
        • Heeg P.
        Reprocessing endoscopes: national recommendations with a special emphasis on cleaning—the German perspective.
        J Hosp Infect. 2004; 56: S23-S26
        • Darbord J.C.
        Importance of cleaning for reprocessing endoscopes and thermolabile sterile medical devices: French use and regulations.
        J Hosp Infect. 2004; 56: S40-S43
        • Kampf G.
        • Grotheer D.
        • Steinmann J.
        Efficacy of three ethanol-based hand rubs against feline calicivirus (FCV).
        J Hosp Infect. 2005; ([in press])